![]() The binding site(s), and the corresponding sequence. Sequences are retrieved from UCSC genome browser, (indicates that the gene has a kB site in the promoter but has not clearly been shown to be controlled by NF-kB or the gene expression is associated with. coli and mammalian cells, and can be used to generate stable clones. pNiFty2-Luc plasmid is selectable with Zeocin in both E. Sequence, from -10 000 to +1000 relatively to the Translation Start pNiFty2-Luc plasmid is composed of three key elements: an ELAM proximal promoter, five NF-B repeated transcription factor binding sites (TFBS) and a Luc (Luciferase) reporter gene. This reporter contains a firefly luciferase gene under the control of multimerized NF-B responsive element located upstream of a minimal promoter. leads to the activation of a select subset of NF-kB/Rel family proteins. The kit contains transfection-ready NF-B luciferase reporter vector. Epstein-Barr Virus Binding to CD21 Activates the Initial Viral Promoter via. Ortholog genes come from the Homologene database. The NF-B Reporter kit is designed for monitoring the activity of the NF-B signaling pathway in the cultured cells. Identifier (PMID) of the associated bibliographical resource, Rabbit), and putative genes in orthologous species. Human genes, checked genes in orthologous species (mouse, rat , pTA-NFkB-luciferase reporter vector, which contains 4 repeats of NFkB binding sites, a minimal promoter upstream of the firefly luciferase coding region. Strong experimental evidence of Rel/NF-kappaB direct control, such asĬhromatin immunoprecipitation or promoter transactivation), putative Groups of genes: Human genes with checked binding sites (there is a TD Gilmore, and additional search with PubMed. This page contains a compilation of Rel/NF-kappaB target genes that isÄerived from the survey paper Activators and target genes of Rel/NF-kappaB transcription factors (Pahl, Oncogene 1999), the Rel/NF-kappaB transcription factors website of The LightSwitch Validated NFkB Pathway Collection is a comprehensive set of sequence-verified, transfection-ready, promoter reporter constructs that showed. Rel/NF-kappaB target genes Rel/NF-kappaB target genes Furthermore, the bi-directional promoter allows the concomitant monitoring of NF-B activation by expression of the two reporters, GFP and luciferase, and enables single-cell analysis of NF-B activation and easier time-resolved analysis. ![]()
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